Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Jianxiong Chan; Gita V Soraya; Lauren Craig; Shah M Uddin; Marian Todaro; Duc H Huynh; Chathurika D Abeyrathne; Lyudmila Kostenko; James McCluskey; Efstratios Skafidas; Patrick Kwan

Journal article

Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Jianxiong Chan, Gita V Soraya, Lauren Craig, Shah M Uddin, Marian Todaro, Duc H Huynh, Chathurika D Abeyrathne, Lyudmila Kostenko, James McCluskey, Efstratios Skafidas, Patrick Kwan

Sensors | MDPI AG | Published : 2019

DOI: 10.3390/s19163543

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Abstract

Pre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to ..

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University of Melbourne Researchers

Marian Todaro Author

James McCluskey Author

Stan Skafidas Author

Patrick Kwan Author

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Grants

Awarded by Australian Research Council

Awarded by Medical Research Future Fund Practitioner Fellowship

Awarded by Australian National Health & Medical Research Council

Awarded by Australian Research Council Discovery Grant ARC Discovery Grant

Funding Acknowledgements

This work was supported in part by the Australian Research Council (DP140101967) and the Royal Melbourne Hospital Neuroscience Foundation. G. V. S. was supported by the Indonesian Endowment Fund for Education. E. S. thanks the generous support of Sue and Leigh Clifford in establishing the Chair in Neural Engineering. P. K. is supported by the Medical Research Future Fund Practitioner Fellowship (1,136,427). J. M. was supported by an Australian National Health & Medical Research Council Program Grant APP1113293 and Australian Research Council Discovery Grant ARC Discovery Grant (DP170103822).